› 7dwa

X-ray diffraction
1.62Å resolution

Structure of a novel beta-mannanase BaMan113A with mannotriose, N236Y mutation

Released:
DOI: 10.2210/pdb7dwa/pdb
PDB entry 7dwa coloured by chain, front view.
PDB entry 7dwa coloured by chain, side view.
PDB entry 7dwa coloured by chain, top view.
Source organism: Bacillus sp. N16-5
Primary publication:
Functional and structural investigation of a novel β-mannanase BaMan113A from Bacillus sp. N16-5.
Liu W, Ma C, Liu W, Zheng Y, Chen CC, Liang A, Luo X, Li Z, Ma W, Song Y, Guo RT, Zhang T
Int J Biol Macromol 182 899-909 (2021)
PMID: 33865894

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102926 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endo-beta-1,4-mannanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 348 amino acids
Theoretical weight: 40.64 KDa
Source organism: Bacillus sp. N16-5
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A140EH91 (Residues: 1-314; Coverage: 100%)
Sequence domains: Glycoside Hydrolase Family 113

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, BMA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Unit cell:
a: 56.114Å b: 109.15Å c: 150.656Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.146 0.175
Expression system: Escherichia coli

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Citations

4 review citations

Stachybotrys chartarum-A Hidden Treasure: Secondary Metabolites, Bioactivities, and Biotechnological Relevance.
Ibrahim et al. (2022)
3 more