› 7e1r

X-ray diffraction
2.8Å resolution

Crystal structure of Dehydrogenase/isomerase FabX from Helicobacter pylori in complex with holo-ACP

Released:
DOI: 10.2210/pdb7e1r/pdb
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PDB entry 7e1r coloured by chain, front view.
PDB entry 7e1r coloured by chain, side view.
PDB entry 7e1r coloured by chain, top view.
Source organism: Helicobacter pylori
Primary publication:
Helicobacter pylori FabX contains a [4Fe-4S] cluster essential for unsaturated fatty acid synthesis.
Zhou J, Zhang L, Zeng L, Yu L, Duan Y, Shen S, Hu J, Zhang P, Song W, Ruan X, Jiang J, Zhang Y, Zhou L, Jia J, Hang X, Tian C, Lin H, Chen HZ, Cronan JE, Bi H, Zhang L
OpenAccess logo Nat Commun 12 6932 (2021)
PMID: 34836944

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110280 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
2-nitropropane dioxygenase Chains: A, C
Molecule details ›
Chains: A, C
Length: 372 amino acids
Theoretical weight: 40.87 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli
UniProt:
  • Canonical: B5Z7D6 (Residues: 1-363; Coverage: 100%)
Gene name: HPG27_730
Sequence domains: Nitronate monooxygenase
Acyl carrier protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 86 amino acids
Theoretical weight: 9.36 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli
UniProt:
  • Canonical: B5Z6T1 (Residues: 1-78; Coverage: 100%)
Gene names: HPG27_519, acpP
Sequence domains: Phosphopantetheine attachment site

Ligands and Environments

3 bound ligands:
Ligand FMN 2 x FMN
Ligand SF4 2 x SF4
Ligand PN7 2 x PN7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 51.793Å b: 104.443Å c: 99.576Å
α: 90° β: 99.52° γ: 90°
R-values:
R R work R free
0.208 0.206 0.243
Expression system: Escherichia coli

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Citations

1 review citation

Unsaturated fatty acid synthesis in bacteria: Mechanisms and regulation of canonical and remarkably noncanonical pathways.
Cronan JE. (2024)
2 other articles

1 mention without citation

Helicobacter pylori FabX contains a [4Fe-4S] cluster essential for unsaturated fatty acid synthesis.
Zhou et al. (2021)

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