› 7ep3

X-ray diffraction
1.51Å resolution

Crystal structure of ZER1 bound to GAGN degron

Released:
DOI: 10.2210/pdb7ep3/pdb
PDB entry 7ep3 coloured by chain, front view.
PDB entry 7ep3 coloured by chain, side view.
PDB entry 7ep3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for recognition of Gly/N-degrons by CRL2ZYG11B and CRL2ZER1.
Yan X, Li Y, Wang G, Zhou Z, Song G, Feng Q, Zhao Y, Mi W, Ma Z, Dong C
Mol Cell 81 3262-3274.e3 (2021)
PMID: 34214466

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182311 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein zer-1 homolog Chain: A
Molecule details ›
Chain: A
Length: 251 amino acids
Theoretical weight: 28.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z7L7 (Residues: 520-766; Coverage: 32%)
Gene names: C9orf60, ZER1, ZYG, ZYG11BL
Sequence domains: Protein zer-1 homolog-like, C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: C2221
Unit cell:
a: 67.97Å b: 120.42Å c: 68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.256
Expression system: Escherichia coli

Quick links

Citations

2 review citations

How the ends signal the end: Regulation by E3 ubiquitin ligases recognizing protein termini.
Sherpa et al. (2022)
1 more

1 mention without citation

CRL2ZER1/ZYG11B recognizes small N-terminal residues for degradation.
Li et al. (2022)