› 7esk

X-ray diffraction
1.05Å resolution

Crystal structure of a L-rhamnose-alpha-1,4-D-glucuronate lyase from Fusarium oxysporum 12S, Ligand free form

Released:
DOI: 10.2210/pdb7esk/pdb
PDB entry 7esk coloured by chain, front view.
PDB entry 7esk coloured by chain, side view.
PDB entry 7esk coloured by chain, top view.
Source organism: Fusarium oxysporum
Primary publication:
Structural and functional analysis of gum arabic l-rhamnose-α-1,4-d-glucuronate lyase establishes a novel polysaccharide lyase family.
Kondo T, Kichijo M, Maruta A, Nakaya M, Takenaka S, Arakawa T, Fushinobu S, Sakamoto T
OpenAccess logo J Biol Chem 297 101001 (2021)
PMID: 34303708

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168246 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
L-Rhamnose-alpha-1,4-D-glucuronate lyase Chain: A
Molecule details ›
Chain: A
Length: 443 amino acids
Theoretical weight: 50.09 KDa
Source organism: Fusarium oxysporum
Expression system: Komagataella pastoris

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand CA 1 x CA
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 58.113Å b: 65.267Å c: 107.008Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.171
Expression system: Komagataella pastoris

Quick links

Citations

1 citations in other articles

Discovery of a novel marine Bacteroidetes with a rich repertoire of carbohydrate-active enzymes.
Chen et al. (2024)

2 mentions without citation

Structural and functional analysis of gum arabic l-rhamnose-α-1,4-d-glucuronate lyase establishes a novel polysaccharide lyase family.
Kondo et al. (2021)
1 more