› 7ey2

X-ray diffraction
2.43Å resolution

Bifunctional xylosidase/glucosidase LXYL D300N mutant with intermediate substrate xylose

Released:
DOI: 10.2210/pdb7ey2/pdb
PDB entry 7ey2 coloured by chain, front view.
PDB entry 7ey2 coloured by chain, side view.
PDB entry 7ey2 coloured by chain, top view.
Source organism: Lentinula edodes
Entry authors: Gong WM, Yang LY

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (7 distinct):
Probable beta-glucosidase G Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 805 amino acids
Theoretical weight: 86.31 KDa
Source organism: Lentinula edodes
Expression system: Escherichia coli
UniProt:
  • Canonical: G8GLP2 (Residues: 1-803; Coverage: 100%)
Gene name: Lxyl-p1-2
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
4 bound ligands:
Ligand NAG 22 x NAG
Ligand XYZ 2 x XYZ
Ligand BMA 1 x BMA
Ligand XYL 3 x XYL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2221
Unit cell:
a: 149.145Å b: 258.225Å c: 320.248Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.226
Expression system: Escherichia coli

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