7f67

Electron Microscopy
3.59Å resolution

eIF2B-SFSV NSs-2-eIF2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
PDBe Complex ID:
PDB-CPX-529185 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Translation initiation factor eIF2B subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 305 amino acids
Theoretical weight: 33.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14232 (Residues: 1-305; Coverage: 100%)
Gene names: EIF2B1, EIF2BA
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 351 amino acids
Theoretical weight: 39.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49770 (Residues: 1-351; Coverage: 100%)
Gene names: EIF2B2, EIF2BB
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit gamma Chains: E, F
Molecule details ›
Chains: E, F
Length: 452 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR50 (Residues: 1-452; Coverage: 100%)
Gene name: EIF2B3
Sequence domains: Nucleotidyl transferase
Translation initiation factor eIF2B subunit delta Chains: G, H
Molecule details ›
Chains: G, H
Length: 523 amino acids
Theoretical weight: 57.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UI10 (Residues: 1-523; Coverage: 100%)
Gene names: EIF2B4, EIF2BD
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit epsilon Chains: I, J
Molecule details ›
Chains: I, J
Length: 721 amino acids
Theoretical weight: 80.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13144 (Residues: 1-721; Coverage: 100%)
Gene names: EIF2B5, EIF2BE
Sequence domains: eIF4-gamma/eIF5/eIF2-epsilon
Non-structural protein NS-S Chains: K, L
Molecule details ›
Chains: K, L
Length: 261 amino acids
Theoretical weight: 29.7 KDa
Source organism: sandfly fever Sicilian virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P12792 (Residues: 7-267; Coverage: 98%)
Gene name: NSS
Sequence domains: Rift valley fever virus non structural protein (NSs) like
Eukaryotic translation initiation factor 2 subunit 1 Chains: N, Q
Molecule details ›
Chains: N, Q
Length: 315 amino acids
Theoretical weight: 36.16 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P05198 (Residues: 1-315; Coverage: 100%)
Gene names: EIF2A, EIF2S1
Sequence domains:
eIF2beta Chains: O, R
Molecule details ›
Chains: O, R
Length: 14 amino acids
Theoretical weight: 1.21 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Eukaryotic translation initiation factor 2 subunit 3 Chains: S, T
Molecule details ›
Chains: S, T
Length: 472 amino acids
Theoretical weight: 51.18 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P41091 (Residues: 1-472; Coverage: 100%)
Gene names: EIF2G, EIF2S3
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.59Å
Relevant EMDB volumes: EMD-31475
Expression systems:
  • Escherichia coli
  • Homo sapiens