7f6g

Electron Microscopy
2.9Å resolution

Cryo-EM structure of human angiotensin receptor AT1R in complex Gq proteins and Sar1-AngII

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-546481 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Angiotensin receptor AT1R Chain: A
Molecule details ›
Chain: A
Length: 725 amino acids
Theoretical weight: 81.57 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
SAR1-AngII Chain: L
Molecule details ›
Chain: L
Length: 8 amino acids
Theoretical weight: 1 KDa
Source organism: Homo sapiens
Expression system: Not provided
Guanine nucleotide-binding protein G(q) subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 369 amino acids
Theoretical weight: 43.53 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P50148 (Residues: 2-359; Coverage: 100%)
Gene names: GAQ, GNAQ
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 354 amino acids
Theoretical weight: 39.09 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 81 amino acids
Theoretical weight: 9.24 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 2-71; Coverage: 99%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-31479
Expression systems:
  • Spodoptera frugiperda
  • Not provided