› 7jhi

X-ray diffraction
2.5Å resolution

Structure of human beta 1,3-N-acetylglucosaminyltransferase 2 iodide-derivative

Released:
DOI: 10.2210/pdb7jhi/pdb
PDB entry 7jhi coloured by chain, front view.
PDB entry 7jhi coloured by chain, side view.
PDB entry 7jhi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures and mechanism of human glycosyltransferase β1,3-N-acetylglucosaminyltransferase 2 (B3GNT2), an important player in immune homeostasis.
Hao Y, Créquer-Grandhomme A, Javier N, Singh A, Chen H, Manzanillo P, Lo MC, Huang X
OpenAccess logo J Biol Chem 296 100042
PMID: 33158990

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192697 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 384 amino acids
Theoretical weight: 44.77 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NY97 (Residues: 14-14, 30-397; Coverage: 93%)
Gene names: B3GALT7, B3GNT1, B3GNT2
Sequence domains: Galactosyltransferase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
6 bound ligands:
Ligand NAG 2 x NAG
Ligand MG 3 x MG
Ligand UDP 2 x UDP
Ligand IOD 41 x IOD
Ligand CL 5 x CL
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 67.431Å b: 81.385Å c: 157.138Å
α: 90° β: 98.63° γ: 90°
R-values:
R R work R free
0.204 0.202 0.248
Expression system: Spodoptera frugiperda

Quick links

Citations

3 review citations

Decoding Strategies to Evade Immunoregulators Galectin-1, -3, and -9 and Their Ligands as Novel Therapeutics in Cancer Immunotherapy.
Lau et al. (2022)
2 more

1 mention without citation

Structures and mechanism of human glycosyltransferase β1,3-N-acetylglucosaminyltransferase 2 (B3GNT2), an important player in immune homeostasis.
Hao et al. (2021)