› 7kma

X-ray diffraction
2.7Å resolution

Crystal structure of eif2Balpha with a ligand.

Released:
DOI: 10.2210/pdb7kma/pdb
PDB entry 7kma coloured by chain, front view.
PDB entry 7kma coloured by chain, side view.
PDB entry 7kma coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Sugar phosphate activation of the stress sensor eIF2B.
Hao Q, Heo JM, Nocek BP, Hicks KG, Stoll VS, Remarcik C, Hackett S, LeBon L, Jain R, Eaton D, Rutter J, Wong YL, Sidrauski C
OpenAccess logo Nat Commun 12 3440 (2021)
PMID: 34103529

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Translation initiation factor eIF2B subunit alpha Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 313 amino acids
Theoretical weight: 34.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14232 (Residues: 1-305; Coverage: 100%)
Gene names: EIF2B1, EIF2BA
Sequence domains: Initiation factor 2 subunit family

Ligands and Environments

2 bound ligands:
Ligand M6P 8 x M6P
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 71.2Å b: 155.54Å c: 140.11Å
α: 90° β: 103.893° γ: 90°
R-values:
R R work R free
0.193 0.191 0.238
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Surviving and Adapting to Stress: Translational Control and the Integrated Stress Response.
Wek et al. (2023)
3 more

1 mention without citation

Sugar phosphate activation of the stress sensor eIF2B.
Hao et al. (2021)