› 7l0f

X-ray diffraction
1.98Å resolution

Monobody 12VC3 Bound to HRAS(WT)

Released:
DOI: 10.2210/pdb7l0f/pdb
PDB entry 7l0f coloured by chain, front view.
PDB entry 7l0f coloured by chain, side view.
PDB entry 7l0f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Selective and noncovalent targeting of RAS mutants for inhibition and degradation.
Teng KW, Tsai ST, Hattori T, Fedele C, Koide A, Yang C, Hou X, Zhang Y, Neel BG, O'Bryan JP, Koide S
OpenAccess logo Nat Commun 12 2656 (2021)
PMID: 33976200

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-524689 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTPase HRas, N-terminally processed Chains: A, E, G, L
Molecule details ›
Chains: A, E, G, L
Length: 167 amino acids
Theoretical weight: 18.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Monobody 12VC3 Chains: B, F, H, M
Molecule details ›
Chains: B, F, H, M
Length: 97 amino acids
Theoretical weight: 10.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand GSP 4 x GSP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 73.012Å b: 64.816Å c: 127.124Å
α: 90° β: 102.37° γ: 90°
R-values:
R R work R free
0.161 0.159 0.191
Expression system: Escherichia coli

Quick links

Citations

16 review citations

KRAS mutation: from undruggable to druggable in cancer.
Huang et al. (2021)
15 more

1 mention without citation

Targeting Ras with protein engineering.
Tomazini et al. (2023)