› 7l0l

Electron Microscopy
2.85Å resolution

Cryo-EM structure of the VRC316 clinical trial, vaccine-elicited, human antibody 316-310-1B11 in complex with an H2 CAN05 HA trimer

Released:
DOI: 10.2210/pdb7l0l/pdb
PDB entry 7l0l coloured by chain, front view.
PDB entry 7l0l coloured by chain, side view.
PDB entry 7l0l coloured by chain, top view.
Source organisms:
Primary publication:
A single residue in influenza virus H2 hemagglutinin enhances the breadth of the B cell response elicited by H2 vaccination.
Andrews SF, Raab JE, Gorman J, Gillespie RA, Cheung CSF, Rawi R, Cominsky LY, Boyington JC, Creanga A, Shen CH, Harris DR, Olia AS, Nazzari AF, Zhou T, Houser KV, Chen GL, Mascola JR, Graham BS, Kanekiyo M, Ledgerwood JE, Kwong PD, McDermott AB
Nat Med 28 373-382 (2022)
PMID: 35115707
Related structures: EMD-23098

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212273 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
316-310-1B11 Light Chain Chains: J, K, L
Molecule details ›
Chains: J, K, L
Length: 215 amino acids
Theoretical weight: 23.46 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Hemagglutinin Chains: A, C, D
Molecule details ›
Chains: A, C, D
Length: 345 amino acids
Theoretical weight: 38.8 KDa
Source organism: Influenza A virus (A/Canada/720/2005(H2N2))
Expression system: Homo sapiens
UniProt:
  • Canonical: Q4ZH98 (Residues: 1-340; Coverage: 61%)
Gene name: HA
Sequence domains: Haemagglutinin
Hemagglutinin HA2 chain Chains: B, E, F
Molecule details ›
Chains: B, E, F
Length: 222 amino acids
Theoretical weight: 25.49 KDa
Source organism: Influenza A virus (A/Canada/720/2005(H2N2))
Expression system: Homo sapiens
UniProt:
  • Canonical: Q4ZH98 (Residues: 341-517; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin
316-310-1B11 Heavy Chain Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 236 amino acids
Theoretical weight: 25.07 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand NAG 15 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.85Å
Relevant EMDB volumes: EMD-23098
Expression system: Homo sapiens

Quick links

Citations

1 review citation

Antibodies to combat viral infections: development strategies and progress.
Pantaleo et al. (2022)
4 other articles