› 7l2e

Electron Microscopy
2.97Å resolution

Cryo-EM structure of NTD-directed neutralizing antibody 4-18 in complex with prefusion SARS-CoV-2 spike glycoprotein

Released:
DOI: 10.2210/pdb7l2e/pdb
PDB entry 7l2e coloured by chain, front view.
PDB entry 7l2e coloured by chain, side view.
PDB entry 7l2e coloured by chain, top view.
Source organisms:
Primary publication:
Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite.
Cerutti G, Guo Y, Zhou T, Gorman J, Lee M, Rapp M, Reddem ER, Yu J, Bahna F, Bimela J, Huang Y, Katsamba PS, Liu L, Nair MS, Rawi R, Olia AS, Wang P, Zhang B, Chuang GY, Ho DD, Sheng Z, Kwong PD, Shapiro L
OpenAccess logo Cell Host Microbe 29 819-833.e7 (2021)
PMID: 33789084
Related structures: EMD-23126

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210211 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Spike glycoprotein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1288 amino acids
Theoretical weight: 142.4 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 1-1208; Coverage: 95%)
Gene names: 2, S
Sequence domains:
4-18 heavy chain Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 229 amino acids
Theoretical weight: 24.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
4-18 light chain Chains: J, K, L
Molecule details ›
Chains: J, K, L
Length: 215 amino acids
Theoretical weight: 23.03 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 30 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.97Å
Relevant EMDB volumes: EMD-23126
Expression system: Homo sapiens

Quick links

Citations

60 review citations

Mechanisms of SARS-CoV-2 entry into cells.
Jackson et al. (2022)
59 more

8 mentions without citation

Structural and functional insights into the spike protein mutations of emerging SARS-CoV-2 variants.
Gupta et al. (2021)
7 more