› 7lat

X-ray diffraction
2.47Å resolution

Campylobacter jejuni keto-acid reductoisomerase in complex with Mg2+

Released:
DOI: 10.2210/pdb7lat/pdb
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PDB entry 7lat coloured by chain, front view.
PDB entry 7lat coloured by chain, side view.
PDB entry 7lat coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Mapping of the Reaction Trajectory catalyzed by Class I Ketol-Acid Reductoisomerase
Lin X, Lonhienne T, Lv Y, Kurz J, McGeary R, Schenk G, Guddat LW
ACS Catalysis (2024)

Function and Biology Details

Reaction catalysed: 
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192896 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ketol-acid reductoisomerase (NADP(+)) Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 35.87 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9PHN5 (Residues: 1-330; Coverage: 97%)
Gene names: Cj0632, ilvC
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: I23
Unit cell:
a: 128.746Å b: 128.746Å c: 128.746Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.159 0.218
Expression system: Escherichia coli

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