› 7lj2

X-ray diffraction
2.4Å resolution

Structure of Exo-L-galactose-6-sulfatase BuS1_11 from Bacteroides uniformis in complex with neoporphyrabiose

Released:
DOI: 10.2210/pdb7lj2/pdb
PDB entry 7lj2 coloured by chain, front view.
PDB entry 7lj2 coloured by chain, side view.
PDB entry 7lj2 coloured by chain, top view.
Source organism: Bacteroides uniformis
Primary publication:
Metabolism of a hybrid algal galactan by members of the human gut microbiome.
Robb CS, Hobbs JK, Pluvinage B, Reintjes G, Klassen L, Monteith S, Giljan G, Amundsen C, Vickers C, Hettle AG, Hills R, Xing X, Montina T, Zandberg WF, Abbott DW, Boraston AB
Nat Chem Biol 18 501-510 (2022)
PMID: 35289327

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-497901 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Acetylglucosamine-6-sulfatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 533 amino acids
Theoretical weight: 62.25 KDa
Source organism: Bacteroides uniformis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A4Y1VMZ7 (Residues: 1-501; Coverage: 100%)
Gene name: Bun01g_38470
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, L6S
Carbohydrate polymer
2 bound ligands:
Ligand CA 2 x CA
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-003
Spacegroup: P41212
Unit cell:
a: 89.67Å b: 89.67Å c: 267.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.23
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structure and function of microbial α-l-fucosidases: a mini review.
Wu et al. (2023)
9 other articles

1 mention without citation

Sulfatases: Critical Enzymes for Algal Polysaccharide Processing.
Hettle et al. (2022)