› 7lk7

X-ray diffraction
2.19Å resolution

Structure of the Exo-alpha-L-galactosidase BpGH29 from Bacteroides plebeius in complex with L-galactose

Released:
DOI: 10.2210/pdb7lk7/pdb
PDB entry 7lk7 coloured by chain, front view.
PDB entry 7lk7 coloured by chain, side view.
PDB entry 7lk7 coloured by chain, top view.
Source organism: Phocaeicola plebeius DSM 17135
Primary publication:
Metabolism of a hybrid algal galactan by members of the human gut microbiome.
Robb CS, Hobbs JK, Pluvinage B, Reintjes G, Klassen L, Monteith S, Giljan G, Amundsen C, Vickers C, Hettle AG, Hills R, Xing X, Montina T, Zandberg WF, Abbott DW, Boraston AB
Nat Chem Biol 18 501-510 (2022)
PMID: 35289327

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-502974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-L-fucosidase C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 599 amino acids
Theoretical weight: 68.35 KDa
Source organism: Phocaeicola plebeius DSM 17135
Expression system: Escherichia coli
UniProt:
  • Canonical: B5CYA5 (Residues: 22-597; Coverage: 100%)
Gene name: BACPLE_01702
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand GXL 1 x GXL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-003
Spacegroup: P21212
Unit cell:
a: 78.156Å b: 132.902Å c: 54.486Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.23
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Sulfatases: Critical Enzymes for Algal Polysaccharide Processing.
Hettle et al. (2022)
1 more

1 mention without citation

Discovery of α-l-Glucosidase Raises the Possibility of α-l-Glucosides in Nature.
Shishiuchi et al. (2022)