› 7mjs

Electron Microscopy
3.03Å resolution

Single-Particle Cryo-EM Structure of Major Facilitator Superfamily Domain containing 2A in complex with LPC-18:3

Released:
DOI: 10.2210/pdb7mjs/pdb
PDB entry 7mjs coloured by chain, front view.
PDB entry 7mjs coloured by chain, side view.
PDB entry 7mjs coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of omega-3 fatty acid transport across the blood-brain barrier.
Cater RJ, Chua GL, Erramilli SK, Keener JE, Choy BC, Tokarz P, Chin CF, Quek DQY, Kloss B, Pepe JG, Parisi G, Wong BH, Clarke OB, Marty MT, Kossiakoff AA, Khelashvili G, Silver DL, Mancia F
Nature 595 315-319 (2021)
PMID: 34135507
Related structures: EMD-23883

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207839 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
2AG3 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 240 amino acids
Theoretical weight: 25.65 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
2AG3 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 23.66 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Sodium-dependent lysophosphatidylcholine symporter 1 Chain: X
Molecule details ›
Chain: X
Length: 546 amino acids
Theoretical weight: 60.79 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: F1NCD6 (Residues: 1-528; Coverage: 100%)
Gene names: MFSD2A, NLS1
Sequence domains: MFS/sugar transport protein

Ligands and Environments

2 bound ligands:
Ligand ZGS 1 x ZGS
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.03Å
Relevant EMDB volumes: EMD-23883
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

10 review citations

Nutrition and mental health: A review of current knowledge about the impact of diet on mental health.
Grajek et al. (2022)
9 more

11 mentions without citation

Label-free visual proteomics: Coupling MS- and EM-based approaches in structural biology.
Klykov et al. (2022)
10 more