› 7mn5

Electron Microscopy
2.93Å resolution

Structure of the HER2/HER3/NRG1b Heterodimer Extracellular Domain

Released:
DOI: 10.2210/pdb7mn5/pdb
PDB entry 7mn5 coloured by chain, front view.
PDB entry 7mn5 coloured by chain, side view.
PDB entry 7mn5 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of the HER2-HER3-NRG1β complex reveal a dynamic dimer interface.
Diwanji D, Trenker R, Thaker TM, Wang F, Agard DA, Verba KA, Jura N
Nature 600 339-343 (2021)
PMID: 34759323
Related structures: EMD-23916

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-250767 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Receptor tyrosine-protein kinase erbB-3 Chain: A
Molecule details ›
Chain: A
Length: 1066 amino acids
Theoretical weight: 117.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P21860 (Residues: 1-1021; Coverage: 76%)
  • Best match: P21860-3 (Residues: 1-330)
Gene names: ERBB3, HER3
Sequence domains:
Neuregulin-1 Chain: H
Molecule details ›
Chain: H
Length: 87 amino acids
Theoretical weight: 9.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02297 (Residues: 176-237; Coverage: 10%)
  • Best match: Q02297-8 (Residues: 176-230)
Gene names: GGF, HGL, HRGA, NDF, NRG1, SMDF
Sequence domains: EGF-like domain
Maltose/maltodextrin-binding periplasmic protein; Receptor tyrosine-protein kinase erbB-2 Chain: B
Molecule details ›
Chain: B
Length: 1455 amino acids
Theoretical weight: 160.5 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P04626 (Residues: 1-1029; Coverage: 82%)
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
Gene names: ERBB2, HER2, JW3994, MLN19, NEU, NGL, b4034, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 5 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.93Å
Relevant EMDB volumes: EMD-23916
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

8 review citations

Resistance to Trastuzumab.
Vivekanandhan et al. (2022)
7 more

7 mentions without citation

Cryo-electron Microscopic Analysis of Single-Pass Transmembrane Receptors.
Cai et al. (2022)
6 more