› 7n5s

X-ray diffraction
2.86Å resolution

ZBTB7A Zinc Finger Domain Bound to -200 Site of Fetal Globin Promoter (Oligo 6)

Released:
DOI: 10.2210/pdb7n5s/pdb
PDB entry 7n5s coloured by chain, front view.
PDB entry 7n5s coloured by chain, side view.
PDB entry 7n5s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for human ZBTB7A action at the fetal globin promoter.
Yang Y, Ren R, Ly LC, Horton JR, Li F, Quinlan KGR, Crossley M, Shi Y, Cheng X
OpenAccess logo Cell Rep 36 109759 (2021)
PMID: 34592153

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-511805 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Zinc finger and BTB domain-containing protein 7A Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95365 (Residues: 369-500; Coverage: 23%)
Gene names: FBI1, LRF, ZBTB7, ZBTB7A, ZNF857A
Sequence domains: Zinc finger, C2H2 type
DNA Strand I Chain: X
Molecule details ›
Chain: X
Length: 15 nucleotides
Theoretical weight: 4.68 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA Strand II Chain: Y
Molecule details ›
Chain: Y
Length: 15 nucleotides
Theoretical weight: 4.51 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P2
Unit cell:
a: 46.938Å b: 36.963Å c: 74.257Å
α: 90° β: 100.012° γ: 90°
R-values:
R R work R free
0.228 0.226 0.272
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

11 citation in other articles

Base-editing-mediated dissection of a γ-globin cis-regulatory element for the therapeutic reactivation of fetal hemoglobin expression.
Antoniou et al. (2022)
10 more

1 mention without citation

Structural basis for human ZBTB7A action at the fetal globin promoter.
Yang et al. (2021)