› 7nin

X-ray diffraction
1.4Å resolution

X-ray crystal structure of LsAA9A - CinnamtanninB1 soak

Released:
DOI: 10.2210/pdb7nin/pdb
PDB entry 7nin coloured by chain, front view.
PDB entry 7nin coloured by chain, side view.
PDB entry 7nin coloured by chain, top view.
Source organism: Panus similis
Primary publication:
Inhibition of lytic polysaccharide monooxygenase by natural plant extracts.
Tokin R, Frandsen KEH, Ipsen JØ, Lo Leggio L, Poojary MM, Berrin JG, Grisel S, Brander S, Jensen PE, Johansen KS
New Phytol 232 1337-1349 (2021)
PMID: 34389999

Function and Biology Details

Reaction catalysed: 
((1->4)-beta-D-glucosyl)(n+m) + reduced acceptor + O(2) = 4-dehydro-beta-D-glucosyl-((1->4)-beta-D-glucosyl)(n-1) + ((1->4)-beta-D-glucosyl)(m) + acceptor + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AA9 family lytic polysaccharide monooxygenase A Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 25.27 KDa
Source organism: Panus similis
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: A0A0S2GKZ1 (Residues: 20-254; Coverage: 100%)
Gene name: LPMO9A
Sequence domains: Auxiliary Activity family 9 (formerly GH61)

Ligands and Environments

5 bound ligands:
Ligand CU 1 x CU
Ligand NAG 1 x NAG
Ligand CL 6 x CL
Ligand UFK 1 x UFK
Ligand ACT 6 x ACT
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P4132
Unit cell:
a: 125.04Å b: 125.04Å c: 125.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.192
Expression system: Aspergillus oryzae

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Citations

4 citation in other articles

A fungal lytic polysaccharide monooxygenase is required for cell wall integrity, thermotolerance, and virulence of the fungal human pathogen Cryptococcus neoformans.
Probst et al. (2023)
3 more