› 7o1z

X-ray diffraction
1.8Å resolution

Unspecific peroxygenase from Hypoxylon sp. EC38 in complex with S-1,2-propanediol

Released:
DOI: 10.2210/pdb7o1z/pdb
PDB entry 7o1z coloured by chain, front view.
PDB entry 7o1z coloured by chain, side view.
PDB entry 7o1z coloured by chain, top view.
Source organism: Hypoxylon sp. EC38
Primary publication:
Structural and biochemical studies enlighten the unspecific peroxygenase from Hypoxylon sp. EC38 as an efficient oxidative biocatalyst.
Rotilio L, Swoboda A, Ebner K, Rinnofner C, Glieder A, Kroutil W, Mattevi A
ACS Catal 11 11511-11525 (2021)
PMID: 34540338

Function and Biology Details

Reaction catalysed: 
RH + H(2)O(2) = ROH + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103895 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme haloperoxidase family profile domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 28.46 KDa
Source organism: Hypoxylon sp. EC38
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A1Y2TH07 (Residues: 1-261; Coverage: 100%)
Gene name: K449DRAFT_467810
Sequence domains: Peroxidase, family 2

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
6 bound ligands:
Ligand PGO 1 x PGO
Ligand NAG 2 x NAG
Ligand PEG 1 x PEG
Ligand 1BO 2 x 1BO
Ligand BCN 1 x BCN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 71.468Å b: 71.468Å c: 152.655Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.124 0.123 0.168
Expression system: Komagataella pastoris

Quick links

Citations

4 review citations

Peroxide-Mediated Oxygenation of Organic Compounds by Fungal Peroxygenases.
Hofrichter et al. (2022)
3 more

1 mention without citation

Comparing the Catalytic and Structural Characteristics of a 'Short' Unspecific Peroxygenase (UPO) Expressed in Pichia pastoris and Escherichia coli.
Robinson et al. (2023)