› 7o2g

X-ray diffraction
2.1Å resolution

Unspecific peroxygenase from Hypoxylon sp. EC38 in complex with styrene

Released:
DOI: 10.2210/pdb7o2g/pdb
PDB entry 7o2g coloured by chain, front view.
PDB entry 7o2g coloured by chain, side view.
PDB entry 7o2g coloured by chain, top view.
Source organism: Hypoxylon sp. EC38
Primary publication:
Structural and biochemical studies enlighten the unspecific peroxygenase from Hypoxylon sp. EC38 as an efficient oxidative biocatalyst.
Rotilio L, Swoboda A, Ebner K, Rinnofner C, Glieder A, Kroutil W, Mattevi A
ACS Catal 11 11511-11525 (2021)
PMID: 34540338

Function and Biology Details

Reaction catalysed: 
RH + H(2)O(2) = ROH + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103895 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme haloperoxidase family profile domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 28.46 KDa
Source organism: Hypoxylon sp. EC38
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A1Y2TH07 (Residues: 1-261; Coverage: 100%)
Gene name: K449DRAFT_467810
Sequence domains: Peroxidase, family 2

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
4 bound ligands:
Ligand NAG 2 x NAG
Ligand DMS 1 x DMS
Ligand SYN 1 x SYN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 71.9Å b: 71.9Å c: 154.555Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.223
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Indigo production goes green: a review on opportunities and challenges of fermentative production.
Chandel et al. (2024)
1 more

1 mention without citation

Peroxide-Mediated Oxygenation of Organic Compounds by Fungal Peroxygenases.
Hofrichter et al. (2022)