› 7o52

X-ray diffraction
2.41Å resolution

CD22 d6-d7 in complex with Fab m971

Released:
DOI: 10.2210/pdb7o52/pdb
PDB entry 7o52 coloured by chain, front view.
PDB entry 7o52 coloured by chain, side view.
PDB entry 7o52 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural details of monoclonal antibody m971 recognition of the membrane-proximal domain of CD22.
Ereño-Orbea J, Liu X, Sicard T, Kucharska I, Li W, Borovsky D, Cui H, Feng Y, Dimitrov DS, Julien JP
OpenAccess logo J Biol Chem 297 100966 (2021)
PMID: 34273351

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-539439 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
m971 Fab Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 229 amino acids
Theoretical weight: 24.47 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
m971 Fab Light chain Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.67 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
B-cell receptor CD22 Chain: U
Molecule details ›
Chain: U
Length: 194 amino acids
Theoretical weight: 21.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P20273 (Residues: 504-687; Coverage: 22%)
Gene names: CD22, SIGLEC2
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 3 x GOL
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: I222
Unit cell:
a: 61.478Å b: 119.719Å c: 241.672Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.264
Expression system: Homo sapiens

Quick links

Citations

1 review citation

Strategies and rules for tuning TCR-derived therapy.
Mo et al. (2023)
7 other articles

1 mention without citation

Structural details of monoclonal antibody m971 recognition of the membrane-proximal domain of CD22.
Ereño-Orbea et al. (2021)