› 7ole

Electron Microscopy
3.41Å resolution

Cryo-EM structure of the TELO2-TTI1-TTI2-RUVBL1-RUVBL2 complex

Released:
DOI: 10.2210/pdb7ole/pdb
PDB entry 7ole coloured by chain, front view.
PDB entry 7ole coloured by chain, side view.
PDB entry 7ole coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the TELO2-TTI1-TTI2 complex and its function in TOR recruitment to the R2TP chaperone.
Pal M, Muñoz-Hernandez H, Bjorklund D, Zhou L, Degliesposti G, Skehel JM, Hesketh EL, Thompson RF, Pearl LH, Llorca O, Prodromou C
OpenAccess logo Cell Rep 36 109317 (2021)
PMID: 34233195
Related structures: EMD-12979

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-519923 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
RuvB-like 1 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 456 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y265 (Residues: 1-456; Coverage: 100%)
Gene names: INO80H, NMP238, RUVBL1, TIP49, TIP49A
Sequence domains:
RuvB-like 2 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 463 amino acids
Theoretical weight: 51.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y230 (Residues: 1-463; Coverage: 100%)
Gene names: CGI-46, INO80J, RUVBL2, TIP48, TIP49B
Sequence domains:
TELO2-interacting protein 1 homolog Chain: H
Molecule details ›
Chain: H
Length: 1733 amino acids
Theoretical weight: 176.84 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: O43156 (Residues: 1-1089; Coverage: 100%)
Gene names: KIAA0406, SMG10, TTI1
Sequence domains: TELO2-interacting protein 1
TELO2-interacting protein 2 Chain: J
Molecule details ›
Chain: J
Length: 964 amino acids
Theoretical weight: 95.63 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q6NXR4 (Residues: 1-508; Coverage: 100%)
Gene names: C8orf41, TTI2
Sequence domains: Tti2 family
Telomere length regulation protein TEL2 homolog Chain: K
Molecule details ›
Chain: K
Length: 491 amino acids
Theoretical weight: 53.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q9Y4R8 (Residues: 1-50, 51-84, 85-161, 162-455; Coverage: 54%)
Gene names: KIAA0683, TELO2

Ligands and Environments

1 bound ligand:
Ligand ADP 5 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.41Å
Relevant EMDB volumes: EMD-12979
Expression systems:
  • Escherichia coli BL21(DE3)
  • Spodoptera aff. frugiperda 1 BOLD-2017

Quick links

Citations

2 review citations

The Role of Hsp90-R2TP in Macromolecular Complex Assembly and Stabilization.
Lynham et al. (2022)
1 more

1 mention without citation

Structure of the TELO2-TTI1-TTI2 complex and its function in TOR recruitment to the R2TP chaperone.
Pal et al. (2021)