› 7ouh

Electron Microscopy
3.5Å resolution

Structure of the STLV intasome:B56 complex bound to the strand-transfer inhibitor bictegravir

Released:
DOI: 10.2210/pdb7ouh/pdb
PDB entry 7ouh coloured by chain, front view.
PDB entry 7ouh coloured by chain, side view.
PDB entry 7ouh coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the inhibition of HTLV-1 integration inferred from cryo-EM deltaretroviral intasome structures.
Barski MS, Vanzo T, Zhao XZ, Smith SJ, Ballandras-Colas A, Cronin NB, Pye VE, Hughes SH, Burke TR, Cherepanov P, Maertens GN
OpenAccess logo Nat Commun 12 4996 (2021)
PMID: 34404793
Related structures: EMD-13077

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121754 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Pol protein Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 301 amino acids
Theoretical weight: 33.94 KDa
Source organism: Simian T-lymphotropic virus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4QY51 (Residues: 600-896; Coverage: 33%)
Gene name: pol
Sequence domains:
PC4 and SFRS1-interacting protein; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform Chains: C, F
Molecule details ›
Chains: C, F
Length: 697 amino acids
Theoretical weight: 80.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75475 (Residues: 1-325; Coverage: 61%)
  • Canonical: Q13362 (Residues: 11-380; Coverage: 71%)
Gene names: DFS70, KIAA0044, LEDGF, PPP2R5C, PSIP1, PSIP2
Sequence domains:
DNA (5'-D(*AP*CP*TP*GP*TP*GP*TP*TP*TP*GP*GP*CP*GP*CP*TP*TP*CP*TP*CP*TP*C)-3') Chains: I, K
Molecule details ›
Chains: I, K
Length: 30 nucleotides
Theoretical weight: 9.22 KDa
DNA (5'-D(*GP*AP*GP*AP*GP*AP*AP*GP*CP*GP*CP*CP*AP*AP*AP*CP*AP*CP*A)-3') Chains: J, L
Molecule details ›
Chains: J, L
Length: 28 nucleotides
Theoretical weight: 8.59 KDa

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand MG 4 x MG
Ligand KLQ 2 x KLQ
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-13077
Expression system: Escherichia coli

Quick links

Citations

1 review citation

New Perspectives about Drug Candidates Targeting HTLV-1 and Related Diseases.
Silva et al. (2023)
2 other articles