› 7oz8

X-ray diffraction
1.95Å resolution

Sulfated host glycan recognition by carbohydrate sulfatases of the human gut microbiota (BT1918_S1_46)

Released:
DOI: 10.2210/pdb7oz8/pdb
PDB entry 7oz8 coloured by chain, front view.
PDB entry 7oz8 coloured by chain, side view.
PDB entry 7oz8 coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Sulfated glycan recognition by carbohydrate sulfatases of the human gut microbiota.
Luis AS, Baslé A, Byrne DP, Wright GSA, London JA, Jin C, Karlsson NG, Hansson GC, Eyers PA, Czjzek M, Barbeyron T, Yates EA, Martens EC, Cartmell A
OpenAccess logo Nat Chem Biol (2022)
PMID: 35710619

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-567781 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfatase N-terminal domain-containing protein Chain: AAA
Molecule details ›
Chain: AAA
Length: 502 amino acids
Theoretical weight: 57.52 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8A6G6 (Residues: 22-500; Coverage: 100%)
Gene name: BT_1918
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand NGS 1 x NGS
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P3221
Unit cell:
a: 56.389Å b: 56.389Å c: 279.763Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.188 0.204
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 review citations

Intestinal mucus and their glycans: A habitat for thriving microbiota.
Luis et al. (2023)
1 more

1 mention without citation

O-Mucin-degrading carbohydrate-active enzymes and their possible implication in inflammatory bowel diseases.
Labourel et al. (2023)