› 7p2p

Electron Microscopy
4.9Å resolution

Human Signal Peptidase Complex Paralog A (SPC-A)

Released:
DOI: 10.2210/pdb7p2p/pdb
PDB entry 7p2p coloured by chain, front view.
PDB entry 7p2p coloured by chain, side view.
PDB entry 7p2p coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human signal peptidase complex reveals the determinants for signal peptide cleavage.
Liaci AM, Steigenberger B, Telles de Souza PC, Tamara S, Gröllers-Mulderij M, Ogrissek P, Marrink SJ, Scheltema RA, Förster F
Mol Cell 81 3934-3948.e11 (2021)
PMID: 34388369
Related structures: EMD-13171

Function and Biology Details

Reaction catalysed: 
Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158171 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Signal peptidase complex catalytic subunit SEC11A Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 22.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P67812 (Residues: 1-179; Coverage: 100%)
Gene names: SEC11A, SEC11L1, SPC18, SPCS4A
Sequence domains: Peptidase S24-like
Signal peptidase complex subunit 3 Chain: B
Molecule details ›
Chain: B
Length: 201 amino acids
Theoretical weight: 22.35 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P61009 (Residues: 1-180; Coverage: 100%)
Gene names: SPC22, SPCS3, UNQ1841/PRO3567
Sequence domains: Signal peptidase subunit
Signal peptidase complex subunit 2 Chain: C
Molecule details ›
Chain: C
Length: 248 amino acids
Theoretical weight: 27.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15005 (Residues: 1-226; Coverage: 100%)
Gene names: KIAA0102, SPC25, SPCS2
Sequence domains: Microsomal signal peptidase 25 kDa subunit (SPC25)
Signal peptidase complex subunit 1 Chain: D
Molecule details ›
Chain: D
Length: 191 amino acids
Theoretical weight: 20.49 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9Y6A9 (Residues: 1-169; Coverage: 100%)
Gene names: HSPC033, SPC12, SPCS1
Sequence domains: Microsomal signal peptidase 12 kDa subunit (SPC12)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.9Å
Relevant EMDB volumes: EMD-13171
Expression system: Homo sapiens

Quick links

Citations

5 review citations

Inhibitors of the Sec61 Complex and Novel High Throughput Screening Strategies to Target the Protein Translocation Pathway.
Pauwels et al. (2021)
4 more