› 7p39

X-ray diffraction
2.9Å resolution

4,6-alpha-glucanotransferase GtfB from Limosilactobacillus reuteri NCC 2613 complexed with acarbose

Released:
DOI: 10.2210/pdb7p39/pdb
PDB entry 7p39 coloured by chain, front view.
PDB entry 7p39 coloured by chain, side view.
PDB entry 7p39 coloured by chain, top view.
Source organism: Limosilactobacillus reuteri
Primary publication:
Insights into Broad-Specificity Starch Modification from the Crystal Structure of Limosilactobacillus Reuteri NCC 2613 4,6-α-Glucanotransferase GtfB.
Pijning T, Gangoiti J, Te Poele EM, Börner T, Dijkhuizen L
OpenAccess logo J Agric Food Chem 69 13235-13245 (2021)
PMID: 34708648

Function and Biology Details

Reaction catalysed: 
Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
dextransucrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 881 amino acids
Theoretical weight: 97.83 KDa
Source organism: Limosilactobacillus reuteri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1Z2RUH3 (Residues: 380-1240; Coverage: 69%)
Sequence domains: Glycosyl hydrolase family 70

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GLC, AC1
Ligand BGC 2 x BGC
Ligand GLC 2 x GLC
Ligand AC1 2 x AC1
2 bound ligands:
Ligand CA 2 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P212121
Unit cell:
a: 107.219Å b: 133.806Å c: 147.866Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.294 0.293 0.315
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Challenges and prospects of microbial α-amylases for industrial application: a review.
Ashok et al. (2023)
2 other articles

1 mention without citation

Insights into Broad-Specificity Starch Modification from the Crystal Structure of Limosilactobacillus Reuteri NCC 2613 4,6-α-Glucanotransferase GtfB.
Pijning et al. (2021)