› 7pbd

Electron Microscopy
3.04Å resolution

a1b3 GABA-A receptor + GABA

Released:
DOI: 10.2210/pdb7pbd/pdb
PDB entry 7pbd coloured by chain, front view.
PDB entry 7pbd coloured by chain, side view.
PDB entry 7pbd coloured by chain, top view.
Source organisms:
Primary publication:
Mechanisms of inhibition and activation of extrasynaptic αβ GABAA receptors.
Kasaragod VB, Mortensen M, Hardwick SW, Wahid AA, Dorovykh V, Chirgadze DY, Smart TG, Miller PS
OpenAccess logo Nature 602 529-533 (2022)
PMID: 35140402
Related structures: EMD-13290

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-537738 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
Gamma-aminobutyric acid receptor subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 368 amino acids
Theoretical weight: 41.99 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P14867 (Residues: 32-339, 418-456; Coverage: 81%)
Gene name: GABRA1
Sequence domains:
Gamma-aminobutyric acid receptor subunit beta-3 Chains: B, C, E
Molecule details ›
Chains: B, C, E
Length: 451 amino acids
Theoretical weight: 51.62 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P28472 (Residues: 33-332, 447-473; Coverage: 73%)
Gene name: GABRB3
Sequence domains:
Gamma-aminobutyric acid receptor subunit alpha-1 Chain: D
Molecule details ›
Chain: D
Length: 411 amino acids
Theoretical weight: 47.06 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P14867 (Residues: 32-339, 418-456; Coverage: 81%)
Gene name: GABRA1
Sequence domains:
Megabody 25 Chain: F
Molecule details ›
Chain: F
Length: 522 amino acids
Theoretical weight: 56.33 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand ABU 2 x ABU
Ligand NAG 3 x NAG
Ligand HSM 1 x HSM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.04Å
Relevant EMDB volumes: EMD-13290
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

9 review citations

Recent Insight into Lipid Binding and Lipid Modulation of Pentameric Ligand-Gated Ion Channels.
Ananchenko et al. (2022)
8 more

1 mention without citation

VHH Nanobody Versatility against Pentameric Ligand-Gated Ion Channels.
Nemecz et al. (2024)