› 7pcq

Electron Microscopy
3.62Å resolution

Human carboxyhemoglobin bound to Staphylococcus aureus hemophore IsdB - 1:1 complex

Released:
DOI: 10.2210/pdb7pcq/pdb
PDB entry 7pcq coloured by chain, front view.
PDB entry 7pcq coloured by chain, side view.
PDB entry 7pcq coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures of staphylococcal IsdB bound to human hemoglobin reveal the process of heme extraction.
De Bei O, Marchetti M, Ronda L, Gianquinto E, Lazzarato L, Chirgadze DY, Hardwick SW, Cooper LR, Spyrakis F, Luisi BF, Campanini B, Bettati S
OpenAccess logo Proc Natl Acad Sci U S A 119 e2116708119 (2022)
PMID: 35357971
Related structures: EMD-13325

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159523 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Hemoglobin subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 141 amino acids
Theoretical weight: 15.15 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P69905 (Residues: 2-142; Coverage: 99%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Hemoglobin subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 146 amino acids
Theoretical weight: 15.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P68871 (Residues: 2-147; Coverage: 99%)
Gene name: HBB
Sequence domains: Globin
Iron-regulated surface determinant protein B Chain: E
Molecule details ›
Chain: E
Length: 372 amino acids
Theoretical weight: 43.39 KDa
Source organism: Staphylococcus aureus subsp. aureus MW2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NX66 (Residues: 124-485; Coverage: 60%)
Gene names: MW1011, frpB, isdB, sasJ, sirH
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand HEM 4 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.62Å
Relevant EMDB volumes: EMD-13325
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Heme acquisition and tolerance in Gram-positive model bacteria: An orchestrated balance.
Wang et al. (2023)
1 more

1 mention without citation

Cryo-EM structures of staphylococcal IsdB bound to human hemoglobin reveal the process of heme extraction.
De Bei et al. (2022)