7pdz

Electron Microscopy
3.8Å resolution

Structure of capping protein bound to the barbed end of a cytoplasmic actin filament

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
PDBe Complex ID:
PDB-CPX-155648 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
F-actin-capping protein subunit beta Chain: E
Molecule details ›
Chain: E
Length: 272 amino acids
Theoretical weight: 30.67 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P47757 (Residues: 1-270; Coverage: 98%)
  • Best match: P47757-2 (Residues: 1-272)
Gene names: Cappb1, Capzb
Sequence domains: F-actin capping protein, beta subunit
F-actin-capping protein subunit alpha-1 Chain: F
Molecule details ›
Chain: F
Length: 286 amino acids
Theoretical weight: 32.98 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P47753 (Residues: 1-286; Coverage: 100%)
Gene names: Cappa1, Capza1
Sequence domains: F-actin capping protein alpha subunit
Actin, cytoplasmic 1 Chains: I, J, K, L, N, O
Molecule details ›
Chains: I, J, K, L, N, O
Length: 375 amino acids
Theoretical weight: 41.8 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P60712 (Residues: 1-375; Coverage: 100%)
Gene name: ACTB
Sequence domains: Actin
Phalloidin Chains: P, Q, R, S, T
Molecule details ›
Chains: P, Q, R, S, T
Length: 7 amino acids
Theoretical weight: 809 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
3 modified residues:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-13343
Expression systems:
  • Escherichia coli
  • Not provided