› 7prs

X-ray diffraction
2Å resolution

Crystal Structure of the B subunit of heat labile enterotoxin LT-IIc from Escherichia coli in complex with Sialyl-lacto-N-neotetraose d

Released:
DOI: 10.2210/pdb7prs/pdb
PDB entry 7prs coloured by chain, front view.
PDB entry 7prs coloured by chain, side view.
PDB entry 7prs coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Characterization of the ganglioside recognition profile of Escherichia coli heat-labile enterotoxin LT-IIc.
Zalem D, Juhás M, Terrinoni M, King-Lyons N, Lebens M, Varrot A, Connell TD, Teneberg S
OpenAccess logo Glycobiology 32 391-403 (2022)
PMID: 34972864

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124849 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Heat-labile enterotoxin IIc B chain Chains: DDD, EEE, FFF, GGG, HHH, III, JJJ, KKK, LLL, MMM
Molecule details ›
Chains: DDD, EEE, FFF, GGG, HHH, III, JJJ, KKK, LLL, MMM
Length: 104 amino acids
Theoretical weight: 11.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: H6W8F2 (Residues: 24-121; Coverage: 100%)
Gene names: GQM13_12885, GQM21_08640, LT-IIc1 B
Sequence domains: Type II heat-labile enterotoxin , B subunit (LT-IIB)

Ligands and Environments

Carbohydrate polymer : NEW Components: SIA, GLC, GAL, NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: SIA, GAL, NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: SIA, GAL, NAG
Carbohydrate polymer
2 bound ligands:
Ligand SO4 4 x SO4
Ligand SIA 4 x SIA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 73.09Å b: 121.269Å c: 126.732Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.23
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

GM1a ganglioside-binding domain peptide inhibits host adhesion and inflammatory response of enterotoxigenic Escherichia coli heat-labile enterotoxin-B in HCT-8 cells.
Park et al. (2023)

1 mention without citation

Characterization of the ganglioside recognition profile of Escherichia coli heat-labile enterotoxin LT-IIc.
Zalem et al. (2022)