› 7q08

Electron Microscopy
2.56Å resolution

Structure of Candida albicans 80S ribosome in complex with cycloheximide

Released:
DOI: 10.2210/pdb7q08/pdb
PDB entry 7q08 coloured by chain, front view.
PDB entry 7q08 coloured by chain, side view.
PDB entry 7q08 coloured by chain, top view.
Source organism: Candida albicans SC5314
Primary publication:
E-site drug specificity of the human pathogen Candida albicans ribosome.
Zgadzay Y, Kolosova O, Stetsenko A, Wu C, Bruchlen D, Usachev K, Validov S, Jenner L, Rogachev A, Yusupova G, Sachs MS, Guskov A, Yusupov M
OpenAccess logo Sci Adv 8 eabn1062 (2022)
PMID: 35613268
Related structures: EMD-13741

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 79-mer (preferred)
PDBe Complex ID:
PDB-CPX-292183 (preferred)
Entry contents:
75 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (79 distinct):
Large ribosomal subunit protein uL2 Chain: j
Molecule details ›
Chain: j
Length: 254 amino acids
Theoretical weight: 27.37 KDa
Source organism: Candida albicans SC5314
UniProt:
  • Canonical: A0A1D8PF08 (Residues: 1-254; Coverage: 100%)
Gene names: CAALFM_C111060CA, RPL2, orf19.2309.2
Sequence domains:
Large ribosomal subunit protein uL3 Chain: k
Molecule details ›
Chain: k
Length: 389 amino acids
Theoretical weight: 44.04 KDa
Source organism: Candida albicans SC5314
UniProt:
  • Canonical: Q59LS1 (Residues: 1-389; Coverage: 100%)
Gene names: CAALFM_C209430WA, RPL3, orf19.1601
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: l
Large ribosomal subunit protein uL18 Chain: m
Large ribosomal subunit protein eL6 Chain: n
Large ribosomal subunit protein uL30 Chain: o
Large ribosomal subunit protein eL8B Chain: p
Large ribosomal subunit protein uL6 Chain: q
Large ribosomal subunit protein uL16 Chain: r
Large ribosomal subunit protein uL5 Chain: s
Large ribosomal subunit protein eL13 Chain: t
Large ribosomal subunit protein eL14 Chain: u
Large ribosomal subunit protein eL15 Chain: v
Large ribosomal subunit protein uL13 Chain: w
Large ribosomal subunit protein uL22 Chain: x
Large ribosomal subunit protein eL18 Chain: y
Large ribosomal subunit protein eL19 Chain: z
Large ribosomal subunit protein eL20 Chain: 0
Large ribosomal subunit protein eL21 Chain: 2
Large ribosomal subunit protein eL22 Chain: 5
Large ribosomal subunit protein uL14 Chain: 6
Large ribosomal subunit protein eL24 Chain: 7
Large ribosomal subunit protein uL23 Chain: 8
Large ribosomal subunit protein uL24 Chain: 9
Large ribosomal subunit protein eL27 Chain: AA
Large ribosomal subunit protein uL15 Chain: AB
Large ribosomal subunit protein eL29 Chain: AC
Large ribosomal subunit protein eL30 Chain: AD
Large ribosomal subunit protein eL31 Chain: AE
Large ribosomal subunit protein eL32 Chain: AF
Large ribosomal subunit protein eL33 Chain: AG
Large ribosomal subunit protein eL34 Chain: AH
Large ribosomal subunit protein uL29 Chain: AI
Large ribosomal subunit protein eL36 Chain: AJ
Large ribosomal subunit protein eL37 Chain: AK
Large ribosomal subunit protein eL38 Chain: AL
Large ribosomal subunit protein eL39 Chain: AM
Large ribosomal subunit protein eL40 Chain: AN
60S ribosomal protein eL41 Chain: AO
Large ribosomal subunit protein eL42 Chain: AP
Large ribosomal subunit protein eL43 Chain: AQ
Ribosome-associated protein STM1 Chain: i
Small ribosomal subunit protein uS2 Chain: B
Small ribosomal subunit protein eS1 Chain: C
Small ribosomal subunit protein uS5 Chain: D
Small ribosomal subunit protein uS3 Chain: E
Small ribosomal subunit protein eS4 Chain: F
Small ribosomal subunit protein uS7 Chain: G
Small ribosomal subunit protein eS6 Chain: H
Small ribosomal subunit protein eS7 Chain: I
Small ribosomal subunit protein eS8 Chain: J
Small ribosomal subunit protein uS4 Chain: K
Small ribosomal subunit protein eS10 Chain: L
Small ribosomal subunit protein uS17 Chain: M
Small ribosomal subunit protein eS12 Chain: N
Small ribosomal subunit protein uS15 Chain: O
Small ribosomal subunit protein uS11 Chain: P
Small ribosomal subunit protein uS19 Chain: Q
Small ribosomal subunit protein uS9 Chain: R
Small ribosomal subunit protein eS17 Chain: S
Small ribosomal subunit protein uS13 Chain: T
Small ribosomal subunit protein eS19 Chain: U
Small ribosomal subunit protein uS10 Chain: V
Small ribosomal subunit protein eS21 Chain: W
Small ribosomal subunit protein uS8A Chain: X
Small ribosomal subunit protein uS12 Chain: Y
Small ribosomal subunit protein eS24 Chain: Z
Small ribosomal subunit protein eS25 Chain: a
Small ribosomal subunit protein eS26 Chain: b
Small ribosomal subunit protein eS27 Chain: c
Small ribosomal subunit protein eS28 Chain: d
Small ribosomal subunit protein uS14 Chain: e
Small ribosomal subunit protein eS30 Chain: f
Ubiquitin-ribosomal protein eS31 fusion protein Chain: g
Small ribosomal subunit protein RACK1 Chain: h
25S ribosomal RNA Chain: 1
5S ribosomal RNA Chain: 3
5.8S ribosomal RNA Chain: 4
18S Ribosomal RNA Chain: A

Ligands and Environments

3 bound ligands:
Ligand SPD 1 x SPD
Ligand 3HE 1 x 3HE
Ligand ZN 8 x ZN
4 modified residues:
Ligand OMG 1 x OMG
Ligand OMC 1 x OMC
Ligand MLZ 3 x MLZ
Ligand IAS 1 x IAS

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.56Å
Relevant EMDB volumes: EMD-13741

Quick links

Citations

2 citation in other articles

Imaging-Based Screening Identifies Modulators of the eIF3 Translation Initiation Factor Complex in Candida albicans.
Metzner et al. (2023)
1 more

1 mention without citation

E-site drug specificity of the human pathogen Candida albicans ribosome.
Zgadzay et al. (2022)