7r81

Electron Microscopy
2.7Å resolution

Structure of the translating Neurospora crassa ribosome arrested by cycloheximide

Released:
Source organism: Neurospora crassa
Related structures: EMD-24307

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 83-mer (preferred)
PDBe Complex ID:
PDB-CPX-244972 (preferred)
Entry contents:
76 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (82 distinct):
Small ribosomal subunit protein uS11 Chain: B2
Molecule details ›
Chain: B2
Length: 290 amino acids
Theoretical weight: 31.53 KDa
Source organism: Neurospora crassa
UniProt:
  • Canonical: Q01291 (Residues: 1-290; Coverage: 100%)
Gene names: B19C19.040, NCU03393, rap-1, rps-0
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein eS1 Chain: C2
Molecule details ›
Chain: C2
Length: 256 amino acids
Theoretical weight: 29.12 KDa
Source organism: Neurospora crassa
UniProt:
  • Canonical: Q871N9 (Residues: 1-256; Coverage: 100%)
Gene names: B11C21.160, NCU01452, crp-8, rps1
Sequence domains: Ribosomal S3Ae family
Large ribosomal subunit protein uL2 C-terminal domain-containing protein Chain: D1
Small ribosomal subunit protein uS5 Chain: D2
Large ribosomal subunit protein uL3 Chain: E1
40S ribosomal protein S3 Chain: E2
Large ribosomal subunit protein uL4 C-terminal domain-containing protein Chain: F1
40S ribosomal protein S4 Chain: F2
Large ribosomal subunit protein uL18 Chain: G1
Small ribosomal subunit protein uS7 Chain: G2
60S ribosomal protein L6 Chain: H1
40S ribosomal protein S6 Chain: H2
Large ribosomal subunit protein uL30 Chain: I1
Small ribosomal subunit protein eS7 Chain: I2
60S ribosomal protein L8 Chain: J1
40S ribosomal protein S8 Chain: J2
Large ribosomal subunit protein uL6 alpha-beta domain-containing protein Chain: K1
40S ribosomal protein S9 Chain: K2
60S ribosomal protein L10 Chain: L1
Probable 40s ribosomal protein s10-b Chain: L2
Large ribosomal subunit protein uL5 Chain: M1
Small ribosomal subunit protein uS17 N-terminal domain-containing protein Chain: M2
60S ribosomal protein L13 Chain: N1
40S ribosomal protein S12 Chain: N2
Large ribosomal subunit protein eL14 domain-containing protein Chain: O1
Small ribosomal subunit protein uS15 N-terminal domain-containing protein Chain: O2
Large ribosomal subunit protein eL15 Chain: P1
40S ribosomal protein S14 (uS11) Chain: P2
60S ribosomal protein L16 Chain: Q1
Ribosomal protein S12 Chain: Q2
Large ribosomal subunit protein uL22 Chain: R1
Small ribosomal subunit protein uS9 Chain: R2
Large ribosomal subunit protein uL15/eL18 domain-containing protein Chain: S1
Small ribosomal subunit protein eS17 Chain: S2
Ribosomal protein L19 Chain: T1
40S ribosomal protein S18 Chain: T2
60S ribosomal protein L20 Chain: U1
40S ribosomal protein S19 Chain: U2
Ribosomal protein Srp1 Chain: V1
Small ribosomal subunit protein uS10 domain-containing protein Chain: V2
60S ribosomal protein L22 Chain: W1
Small ribosomal subunit protein eS21 Chain: W2
60S ribosomal protein L23 Chain: X1
Small ribosomal subunit protein uS8 Chain: X2
Large ribosomal subunit protein eL24 Chain: Y1
Small ribosomal subunit protein uS12 Chain: Y2
Large ribosomal subunit protein uL23 N-terminal domain-containing protein Chain: Z1
40S ribosomal protein S24 Chain: Z2
KOW domain-containing protein Chain: a1
Small ribosomal subunit protein eS25 Chain: a2
60S ribosomal protein L27 Chain: b1
Small ribosomal subunit protein eS26 Chain: b2
Large ribosomal subunit protein uL15 Chain: c1
Small ribosomal subunit protein eS27 Chain: c2
60S ribosomal protein L29 Chain: d1
Small ribosomal subunit protein eS28 Chain: d2
Large ribosomal subunit protein eL30 Chain: e1
Small ribosomal subunit protein uS14 Chain: e2
60s ribosomal protein Chain: f1
40S ribosomal protein S30 Chain: f2
Large ribosomal subunit protein eL32 Chain: g1
Ubiquitin-ribosomal protein eS31 fusion protein Chain: g2
60S ribosomal protein L33 Chain: h1
Small ribosomal subunit protein RACK1 Chain: h2
60S ribosomal protein L34 Chain: i1
60S ribosomal protein L35 Chain: j1
60S ribosomal protein L36 Chain: k1
Ribosomal protein L37 Chain: l1
Large ribosomal subunit protein eL38 Chain: m1
Large ribosomal subunit protein eL39 Chain: n1
Large ribosomal subunit protein eL40 domain-containing protein Chain: o1
60S ribosomal protein L41 Chain: p1
60S ribosomal protein L44 Chain: q1
60S ribosomal protein L43 Chain: r1
Ribosomal eL28/Mak16 domain-containing protein Chain: s1
Nascent peptide Chain: v1
26S rRNA Chain: A1
18S Ribosomal RNA Chain: A2
5S ribosomal RNA Chain: B1
5.8S ribosomal RNA Chain: C1
tRNA Chains: t1, u1
Messenger RNA Chain: w1

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-24307