› 7rjo

X-ray diffraction
1.38Å resolution

Crystal structure of human Bromodomain containing protein 4 (BRD4) in complex with hnRNPK

Released:
DOI: 10.2210/pdb7rjo/pdb
PDB entry 7rjo coloured by chain, front view.
PDB entry 7rjo coloured by chain, side view.
PDB entry 7rjo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Uncovering the Bromodomain Interactome using Site-Specific Azide-Acetyllysine Photochemistry, Proteomic Profiling and Structural Characterization
Wagner S, Fedorov E, Sudhamalla B, Jnawali HN, Debiec R, Ghosh A, Islam K
Biorxiv (2021)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-521196 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bromodomain-containing protein 4 Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 15.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60885 (Residues: 44-168; Coverage: 9%)
Gene names: BRD4, HUNK1
Sequence domains: Bromodomain
Heterogeneous nuclear ribonucleoprotein K Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.1 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P61978 (Residues: 57-66; Coverage: 2%)
Gene names: HNRNPK, HNRPK

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand GOL 2 x GOL
Ligand ACT 2 x ACT
1 modified residue:
Ligand ALY 2 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P212121
Unit cell:
a: 48.454Å b: 52.08Å c: 53.325Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.198
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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