› 7s2s

X-ray diffraction
1.93Å resolution

nanobody bound to Interleukin-2Rbeta

Released:
DOI: 10.2210/pdb7s2s/pdb
PDB entry 7s2s coloured by chain, front view.
PDB entry 7s2s coloured by chain, side view.
PDB entry 7s2s coloured by chain, top view.
Source organisms:
Primary publication:
Facile discovery of surrogate cytokine agonists.
Yen M, Ren J, Liu Q, Glassman CR, Sheahan TP, Picton LK, Moreira FR, Rustagi A, Jude KM, Zhao X, Blish CA, Baric RS, Su LL, Garcia KC
Cell 185 1414-1430.e19 (2022)
PMID: 35325595

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210608 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
IL2Rb-binding nanobody Chains: A, C
Molecule details ›
Chains: A, C
Length: 154 amino acids
Theoretical weight: 16.58 KDa
Source organism: Camelus bactrianus
Expression system: Homo sapiens
Interleukin-2 receptor subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 216 amino acids
Theoretical weight: 25.2 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P14784 (Residues: 27-236; Coverage: 40%)
Gene names: IL15RB, IL2RB
Sequence domains: Interleukin-2 receptor subunit beta N-terminal domain 1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand SO4 13 x SO4
1 modified residue:
Ligand MLY 6 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P1
Unit cell:
a: 42.698Å b: 69.505Å c: 91.721Å
α: 99.566° β: 99.854° γ: 90.191°
R-values:
R R work R free
0.214 0.213 0.247
Expression system: Homo sapiens

Quick links

Citations

7 review citations

The JAK-STAT pathway at 30: Much learned, much more to do.
Philips et al. (2022)
6 more

2 mentions without citation

Emerging principles of cytokine pharmacology and therapeutics.
Saxton et al. (2023)
1 more