› 7t25

X-ray diffraction
2.25Å resolution

OspA-Fab 319-44 complex structure

Released:
DOI: 10.2210/pdb7t25/pdb
PDB entry 7t25 coloured by chain, front view.
PDB entry 7t25 coloured by chain, side view.
PDB entry 7t25 coloured by chain, top view.
Source organisms:
Entry author: Rudolph MJ

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-534030 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
319-44 Fab heavy chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 223 amino acids
Theoretical weight: 24.19 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
319-44 Fab light chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 215 amino acids
Theoretical weight: 23.3 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Outer surface protein A Chains: C, E
Molecule details ›
Chains: C, E
Length: 256 amino acids
Theoretical weight: 27.6 KDa
Source organism: Borreliella burgdorferi B31
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CL66 (Residues: 18-273; Coverage: 100%)
Gene names: BB_A15, ospA
Sequence domains: Borrelia lipoprotein

Ligands and Environments

4 bound ligands:
Ligand CL 29 x CL
Ligand NAG 2 x NAG
Ligand IOD 5 x IOD
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 254.208Å b: 44.117Å c: 143.219Å
α: 90° β: 101.23° γ: 90°
R-values:
R R work R free
0.193 0.191 0.233
Expression systems:
  • Cricetulus griseus
  • Escherichia coli BL21(DE3)

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