› 7toh

X-ray diffraction
1.26Å resolution

Crystal structure of carbohydrate esterase PbeAcXE, in complex with MeGlcpA-Xylp

Released:
DOI: 10.2210/pdb7toh/pdb
PDB entry 7toh coloured by chain, front view.
PDB entry 7toh coloured by chain, side view.
PDB entry 7toh coloured by chain, top view.
Source organism: Prolixibacter bellariivorans
Primary publication:
Elucidating Sequence and Structural Determinants of Carbohydrate Esterases for Complete Deacetylation of Substituted Xylans.
Penttinen L, Kouhi V, Fauré R, Skarina T, Stogios P, Master E, Jurak E
OpenAccess logo Molecules 27 (2022)
PMID: 35566004

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105439 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
SGNH hydrolase-type esterase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 42.73 KDa
Source organism: Prolixibacter bellariivorans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A5M4AV20 (Residues: 21-404; Coverage: 100%)
Gene name: PbJCM13498_02590
Sequence domains: GDSL-like Lipase/Acylhydrolase family

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP, GCV
Ligand XYP 1 x XYP
Ligand GCV 1 x GCV
No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 57.61Å b: 42.81Å c: 76.7Å
α: 90° β: 108.44° γ: 90°
R-values:
R R work R free
0.136 0.135 0.169
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 mentions without citation

Highly accurate carbohydrate-binding site prediction with DeepGlycanSite.
He et al. (2024)
1 more