› 7uja

Electron Microscopy
3.7Å resolution

Cryo-EM structure of Human respiratory syncytial virus F variant (construct pXCS847A)

Released:
DOI: 10.2210/pdb7uja/pdb
PDB entry 7uja coloured by chain, front view.
PDB entry 7uja coloured by chain, side view.
PDB entry 7uja coloured by chain, top view.
Source organisms:
Primary publication:
Rational Design of a Highly Immunogenic Prefusion-Stabilized F Glycoprotein Antigen for a Respiratory Syncytial Virus Vaccine.
Che Y, Gribenko AV, Song X, Handke LD, Efferen KS, Tompkins K, Kodali S, Nunez L, Prasad AK, Phelan LM, Ammirati M, Yu X, Lees JA, Chen W, Martinez L, Roopchand V, Han S, Qiu X, DeVincenzo JP, Jansen KU, Dormitzer PR, Swanson KA
Sci Transl Med eade6422 (2023)
PMID: 37023209
Related structures: EMD-26562

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-496285 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Fusion glycoprotein F0 Chains: A, C, K
Molecule details ›
Chains: A, C, K
Length: 410 amino acids
Theoretical weight: 45.03 KDa
Source organism: Respiratory syncytial virus
Expression system: Mammalia
UniProt:
  • Canonical: A0A218N9U8 (Residues: 137-513; Coverage: 66%)
Sequence domains: Fusion glycoprotein F0
AM14 Fab heavy chain Chains: B, G, L
Molecule details ›
Chains: B, G, L
Length: 245 amino acids
Theoretical weight: 26.23 KDa
Source organism: Mus musculus
Expression system: Mammalia
AM14 Fab light chain Chains: D, H, M
Molecule details ›
Chains: D, H, M
Length: 218 amino acids
Theoretical weight: 23.97 KDa
Source organism: Mus musculus
Expression system: Mammalia
AM22 Fab heavy chain Chains: E, I, N
Molecule details ›
Chains: E, I, N
Length: 246 amino acids
Theoretical weight: 25.99 KDa
Source organism: Mus musculus
Expression system: Mammalia
AM22 Fab light chain Chains: F, J, O
Molecule details ›
Chains: F, J, O
Length: 215 amino acids
Theoretical weight: 23.27 KDa
Source organism: Mus musculus
Expression system: Mammalia
Fusion glycoprotein F0 Chains: P, Q, R
Molecule details ›
Chains: P, Q, R
Length: 109 amino acids
Theoretical weight: 12.11 KDa
Source organism: Respiratory syncytial virus
Expression system: Mammalia
UniProt:
  • Canonical: E0WLS1 (Residues: 1-1, 4-109; Coverage: 65%)
Gene name: F
Sequence domains: Fusion glycoprotein F0

Ligands and Environments

1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-26562
Expression system: Mammalia

Quick links

Citations

7 review citations

The road to approved vaccines for respiratory syncytial virus.
Ruckwardt TJ. (2023)
6 more

2 mentions without citation

Proscan: a structure-based proline design web server.
Felbinger et al. (2024)
1 more