› 7uov

Electron Microscopy
2.75Å resolution

Native Lassa glycoprotein in complex with neutralizing antibodies 12.1F and 37.2D

Released:
DOI: 10.2210/pdb7uov/pdb
PDB entry 7uov coloured by chain, front view.
PDB entry 7uov coloured by chain, side view.
PDB entry 7uov coloured by chain, top view.
Source organisms:
Primary publication:
A cocktail of protective antibodies subverts the dense glycan shield of Lassa virus.
Li H, Buck T, Zandonatti M, Yin J, Moon-Walker A, Fang J, Koval A, Heinrich ML, Rowland MM, Diaz Avalos R, Schendel SL, Parekh D, Zyla D, Enriquez A, Harkins S, Sullivan B, Smith V, Chukwudozie O, Watanabe R, Robinson JE, Garry RF, Branco LM, Hastie KM, Saphire EO
Sci Transl Med 14 eabq0991 (2022)
PMID: 36288283
Related structures: EMD-26655

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-225107 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (12 distinct):
12.1F heavy chain (variable domain) Chains: D, F, H
Molecule details ›
Chains: D, F, H
Length: 114 amino acids
Theoretical weight: 12.73 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
12.1F light chain (variable domain) Chains: E, G, L
Molecule details ›
Chains: E, G, L
Length: 106 amino acids
Theoretical weight: 11.6 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
Glycoprotein G1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 259 amino acids
Theoretical weight: 29.06 KDa
Source organism: Lassa virus Josiah
Expression system: Homo sapiens
UniProt:
  • Canonical: P08669 (Residues: 1-259; Coverage: 53%)
Gene names: GP-C, GPC
Sequence domains: Arenavirus glycoprotein
Glycoprotein G2 Chains: a, b, c
Molecule details ›
Chains: a, b, c
Length: 232 amino acids
Theoretical weight: 26.8 KDa
Source organism: Lassa virus Josiah
Expression system: Homo sapiens
UniProt:
  • Canonical: P08669 (Residues: 260-491; Coverage: 47%)
Gene names: GP-C, GPC
Sequence domains: Arenavirus glycoprotein
37.2D light chain (variable domain) Chains: P, R, T
Molecule details ›
Chains: P, R, T
Length: 105 amino acids
Theoretical weight: 11.41 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
37.2D heavy chain (variable domain) Chains: O, Q, S
Molecule details ›
Chains: O, Q, S
Length: 126 amino acids
Theoretical weight: 13.95 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Ligand NAG 12 x NAG
Ligand BMA 6 x BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Ligand NAG 12 x NAG
Ligand BMA 6 x BMA
Ligand MAN 18 x MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Ligand NAG 4 x NAG
Ligand BMA 2 x BMA
Ligand MAN 2 x MAN
Ligand FUC 2 x FUC
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Ligand NAG 14 x NAG
Ligand BMA 7 x BMA
Ligand FUC 7 x FUC
Carbohydrate polymer : NEW Components: NAG
Ligand NAG 16 x NAG
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Ligand NAG 6 x NAG
Ligand BMA 3 x BMA
Ligand MAN 6 x MAN
Ligand FUC 3 x FUC
1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.75Å
Relevant EMDB volumes: EMD-26655
Expression systems:
  • Drosophila melanogaster
  • Homo sapiens

Quick links

Citations

1 review citation

Uses and Challenges of Antiviral Polyclonal and Monoclonal Antibody Therapies.
Struble et al. (2023)
1 other article

2 mentions without citation

A cocktail of protective antibodies subverts the dense glycan shield of Lassa virus.
Li et al. (2022)
1 more