› 7usr

X-ray diffraction
1.93Å resolution

Plasmodium falciparum protein Pfs230 D1D2 - Structure of the first two 6-cysteine domains

Released:
DOI: 10.2210/pdb7usr/pdb
PDB entry 7usr coloured by chain, front view.
PDB entry 7usr coloured by chain, side view.
PDB entry 7usr coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
Nanobodies against Pfs230 block Plasmodium falciparum transmission.
Dietrich MH, Gabriela M, Reaksudsan K, Dixon MWA, Chan LJ, Adair A, Trickey S, O'Neill MT, Tan LL, Lopaticki S, Healer J, Keremane S, Cowman AF, Tham WH
OpenAccess logo Biochem J 479 2529-2546 (2022)
PMID: 36520108

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159527 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Gametocyte surface protein P230 Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 35.41 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P68874 (Residues: 587-889; Coverage: 10%)
Gene names: PF230, PF3D7_0209000, PFS230, S230
Sequence domains: Sexual stage antigen s48/45 domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Ligand NAG 2 x NAG
Ligand FUC 1 x FUC
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P43
Unit cell:
a: 65.788Å b: 65.788Å c: 108.035Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.205
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Nanobodies: A Review of Generation, Diagnostics and Therapeutics.
Jin et al. (2023)
1 more

1 mention without citation

Nanobodies against Pfs230 block Plasmodium falciparum transmission.
Dietrich et al. (2022)