› 7vkt

Electron Microscopy
2.9Å resolution

cryo-EM structure of LTB4-bound BLT1 in complex with Gi protein

Released:
DOI: 10.2210/pdb7vkt/pdb
PDB entry 7vkt coloured by chain, front view.
PDB entry 7vkt coloured by chain, side view.
PDB entry 7vkt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of leukotriene B4 receptor 1 activation.
Wang N, He X, Zhao J, Jiang H, Cheng X, Xia Y, Eric Xu H, He Y
OpenAccess logo Nat Commun 13 1156 (2022)
PMID: 35241677
Related structures: EMD-32018

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-548178 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Leukotriene B4 receptor 1 Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 37.74 KDa
Source organism: Homo sapiens
Expression system: Insect BA phytoplasma
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: B
Molecule details ›
Chain: B
Length: 354 amino acids
Theoretical weight: 40.41 KDa
Source organism: Homo sapiens
Expression system: Insect BA phytoplasma
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Insect BA phytoplasma
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Insect BA phytoplasma
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 247 amino acids
Theoretical weight: 26.28 KDa
Source organism: Homo sapiens
Expression system: Insect BA phytoplasma

Ligands and Environments

3 bound ligands:
Ligand LTB 1 x LTB
Ligand Y01 4 x Y01
Ligand 8IO 1 x 8IO
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-32018
Expression system: Insect BA phytoplasma

Quick links

Citations

3 review citations

The Role of G Protein-Coupled Receptor Kinase 6 Regulation in Inflammation and Pain.
Stegen et al. (2022)
2 more

5 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
4 more