› 7vn5

X-ray diffraction
1.95Å resolution

Crystal structure of MBP-fused BIL1/BZR1 (21-90) in complex with double-stranded DNA contaning TTCACGTGAA

Released:
DOI: 10.2210/pdb7vn5/pdb
PDB entry 7vn5 coloured by chain, front view.
PDB entry 7vn5 coloured by chain, side view.
PDB entry 7vn5 coloured by chain, top view.
Source organisms:
Primary publication:
Brassinosteroid-induced gene repression requires specific and tight promoter binding of BIL1/BZR1 via DNA shape readout.
Nosaki S, Mitsuda N, Sakamoto S, Kusubayashi K, Yamagami A, Xu Y, Bui TBC, Terada T, Miura K, Nakano T, Tanokura M, Miyakawa T
Nat Plants (2022)
PMID: 36522451

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-221100 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (3 distinct):
Maltose/maltodextrin-binding periplasmic protein; Protein BRASSINAZOLE-RESISTANT 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 439 amino acids
Theoretical weight: 48.42 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-384, 387-387, 391-393; Coverage: 98%)
  • Canonical: Q8S307 (Residues: 11-12, 14-16, 20-90; Coverage: 23%)
Gene names: At1g75080, BIS2, BZR1, F9E10.7, JW3994, b4034, malE
Sequence domains:
DNA (5'-D(*TP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*AP*A)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 15 nucleotides
Theoretical weight: 4.59 KDa

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand EDO 13 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21
Unit cell:
a: 102.622Å b: 93.923Å c: 112.75Å
α: 90° β: 100.646° γ: 90°
R-values:
R R work R free
0.211 0.209 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Shaping transcriptional responses to a phytohormone.
Favero DS. (2023)
1 more