› 7vsh

Electron Microscopy
3.4Å resolution

Cryo-EM structure of a human ATP11C-CDC50A flippase reconstituted in the Nanodisc in E1P state.

Released:
DOI: 10.2210/pdb7vsh/pdb
PDB entry 7vsh coloured by chain, front view.
PDB entry 7vsh coloured by chain, side view.
PDB entry 7vsh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM of the ATP11C flippase reconstituted in Nanodiscs shows a distended phospholipid bilayer inner membrane around transmembrane helix 2.
Nakanishi H, Hayashida K, Nishizawa T, Oshima A, Abe K
OpenAccess logo J Biol Chem 298 101498 (2022)
PMID: 34922944
Related structures: EMD-32111

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185461 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Phospholipid-transporting ATPase IG Chain: A
Molecule details ›
Chain: A
Length: 1084 amino acids
Theoretical weight: 124.4 KDa
Source organism: Homo sapiens
Expression system: Mammalia
UniProt:
  • Canonical: Q8NB49 (Residues: 13-1094; Coverage: 96%)
Gene names: ATP11C, ATPIG, ATPIQ
Sequence domains:
Cell cycle control protein 50A Chain: C
Molecule details ›
Chain: C
Length: 361 amino acids
Theoretical weight: 40.73 KDa
Source organism: Homo sapiens
Expression system: Mammalia
UniProt:
  • Canonical: Q9NV96 (Residues: 1-361; Coverage: 100%)
Gene names: C6orf67, CDC50A, TMEM30A
Sequence domains: LEM3 (ligand-effect modulator 3) family / CDC50 family

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
4 bound ligands:
Ligand MG 1 x MG
Ligand ALF 1 x ALF
Ligand 17F 1 x 17F
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-32111
Expression system: Mammalia

Quick links

Citations

1 review citation

Physiological and Pathological Functions of TMEM30A: An Essential Subunit of P4-ATPase Phospholipid Flippases.
Li et al. (2023)