› 7wdn

X-ray diffraction
1.8Å resolution

Crystal structures of MeBglD2 in complex with various saccharides

Released:
DOI: 10.2210/pdb7wdn/pdb
PDB entry 7wdn coloured by chain, front view.
PDB entry 7wdn coloured by chain, side view.
PDB entry 7wdn coloured by chain, top view.
Source organism: metagenome
Primary publication:
Crystal structure of metagenomic β-glycosidase MeBglD2 in complex with various saccharides.
Matsuzawa T, Watanabe M, Nakamichi Y, Akita H, Yaoi K
Appl Microbiol Biotechnol 106 4539-4551 (2022)
PMID: 35723691

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103422 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 457 amino acids
Theoretical weight: 52.09 KDa
Source organism: metagenome
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1E1FFN6 (Residues: 2-455; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 1

Ligands and Environments

1 bound ligand:
Ligand GLC 4 x GLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: I432
Unit cell:
a: 202.488Å b: 202.488Å c: 202.488Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.159 0.21
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Structural and mutational analysis of glycoside hydrolase family 1 Br2 β-glucosidase derived from bovine rumen metagenome.
Kaenying et al. (2023)