› 7wj9

X-ray diffraction
1.75Å resolution

Crystal structure of Lactococcus lactis subsp. cremoris GH31 alpha-1,3-glucosidase, P21 space group

Released:
DOI: 10.2210/pdb7wj9/pdb
PDB entry 7wj9 coloured by chain, front view.
PDB entry 7wj9 coloured by chain, side view.
PDB entry 7wj9 coloured by chain, top view.
Source organism: Lactococcus lactis subsp. cremoris MG1363
Primary publication:
Structural basis of the strict specificity of a bacterial GH31 α-1,3-glucosidase for nigerooligosaccharides.
Ikegaya M, Moriya T, Adachi N, Kawasaki M, Park EY, Miyazaki T
OpenAccess logo J Biol Chem 298 101827 (2022)
PMID: 35293315

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106950 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-xylosidase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 759 amino acids
Theoretical weight: 88.03 KDa
Source organism: Lactococcus lactis subsp. cremoris MG1363
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A2RM80 (Residues: 1-739; Coverage: 100%)
Gene names: llmg_1836, xylS
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand XYL 6 x XYL
Ligand EDO 14 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 115.4Å b: 207.853Å c: 117.899Å
α: 90° β: 103.63° γ: 90°
R-values:
R R work R free
0.187 0.186 0.217
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

Mechanism of high-mannose N-glycan breakdown and metabolism by Bifidobacterium longum.
Cordeiro et al. (2023)
1 more

2 mentions without citation

Structural basis of the strict specificity of a bacterial GH31 α-1,3-glucosidase for nigerooligosaccharides.
Ikegaya et al. (2022)
1 more