› 7wyz

Electron Microscopy
3.4Å resolution

Cryo-EM structure of Na+,K+-ATPase in the E2P state formed by ATP with ouabain

Released:
DOI: 10.2210/pdb7wyz/pdb
PDB entry 7wyz coloured by chain, front view.
PDB entry 7wyz coloured by chain, side view.
PDB entry 7wyz coloured by chain, top view.
Source organism: Squalus acanthias
Primary publication:
Cryoelectron microscopy of Na+,K+-ATPase in the two E2P states with and without cardiotonic steroids.
Kanai R, Cornelius F, Vilsen B, Toyoshima C
OpenAccess logo Proc Natl Acad Sci U S A 119 e2123226119 (2022)
PMID: 35380894
Related structures: EMD-32899

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111292 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (7 distinct):
Sodium/potassium-transporting ATPase subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 1028 amino acids
Theoretical weight: 113.39 KDa
Source organism: Squalus acanthias
UniProt:
  • Canonical: Q4H132 (Residues: 1-1028; Coverage: 100%)
Gene name: atn1
Sequence domains:
Sodium/potassium-transporting ATPase subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 305 amino acids
Theoretical weight: 35.18 KDa
Source organism: Squalus acanthias
UniProt:
  • Canonical: C4IX13 (Residues: 1-305; Coverage: 100%)
Gene name: atp1b1
Sequence domains: Sodium / potassium ATPase beta chain
FXYD domain-containing ion transport regulator Chains: E, G
Molecule details ›
Chains: E, G
Length: 94 amino acids
Theoretical weight: 10.2 KDa
Source organism: Squalus acanthias
UniProt:
  • Canonical: Q70Q12 (Residues: 1-94; Coverage: 100%)
Gene name: fxyd10
Sequence domains: ATP1G1/PLM/MAT8 family

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand MG 2 x MG
Ligand NA 4 x NA
Ligand OBN 2 x OBN
Ligand CLR 8 x CLR
Ligand PCW 24 x PCW
1 modified residue:
Ligand PHD 2 x PHD

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-32899

Quick links

Citations

4 citation in other articles

Role of the Rhamnosyl Residue of Ouabain in the Activation of the Na,K-ATPase Signaling Function.
Rogachevskii et al. (2023)
3 more