7x24

Electron Microscopy
3.4Å resolution

Cryo-EM structure of non gastric H,K-ATPase alpha2 SPWC mutant in (2K+)E2-AlF state

Released:
Source organism: Rattus norvegicus
Related structures: EMD-32957

Function and Biology Details

Reactions catalysed:
ATP + H(2)O + Na(+)(Side 1) + K(+)(Side 2) = ADP + phosphate + Na(+)(Side 2) + K(+)(Side 1)
ATP + H(2)O + H(+)(Side 1) + K(+)(Side 2) = ADP + phosphate + H(+)(Side 2) + K(+)(Side 1)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-139526 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Potassium-transporting ATPase alpha chain 2 Chain: A
Molecule details ›
Chain: A
Length: 986 amino acids
Theoretical weight: 109.15 KDa
Source organism: Rattus norvegicus
Expression system: Homo sapiens
UniProt:
  • Canonical: P54708 (Residues: 53-1036; Coverage: 95%)
  • Best match: P54708-2 (Residues: 1-928)
Gene names: Atp12a, Atp1al1
Sequence domains:
Sodium/potassium-transporting ATPase subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 324 amino acids
Theoretical weight: 37.52 KDa
Source organism: Rattus norvegicus
Expression system: Homo sapiens
UniProt:
  • Canonical: P07340 (Residues: 1-304; Coverage: 100%)
Gene name: Atp1b1
Sequence domains: Sodium / potassium ATPase beta chain

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-32957
Expression system: Homo sapiens