› 7xdq

X-ray diffraction
2.83Å resolution

Crystal structure of a glucosylglycerol phosphorylase mutant from Marinobacter adhaerens

Released:
DOI: 10.2210/pdb7xdq/pdb
PDB entry 7xdq coloured by chain, front view.
PDB entry 7xdq coloured by chain, side view.
PDB entry 7xdq coloured by chain, top view.
Source organism: Marinobacter adhaerens
Primary publication:
Protein Engineering of Glucosylglycerol Phosphorylase Facilitating Efficient and Highly Regio- and Stereoselective Glycosylation of Polyols in a Synthetic System.
Zhang T, Liu P, Wei H, Sun X, Zeng Y, Zhang X, Cai Y, Cui M, Ma H, Liu W, Sun Y, Yang J
ACS Catalysis 15715-15727 (2022)

Function and Biology Details

Reaction catalysed: 
2-O-alpha-D-glucopyranosyl-glycerol + phosphate = alpha-D-glucose 1-phosphate + glycerol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-513869 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosylglycerol phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 480 amino acids
Theoretical weight: 54.93 KDa
Source organism: Marinobacter adhaerens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E4PMA5 (Residues: 1-480; Coverage: 100%)
Gene names: HP15_2853, gtfA
Sequence domains: Alpha amylase, catalytic domain

Ligands and Environments

2 bound ligands:
Ligand BGC 1 x BGC
Ligand LI 1 x LI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL02U1
Spacegroup: R32
Unit cell:
a: 176.437Å b: 176.437Å c: 128.473Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.244 0.302
Expression system: Escherichia coli BL21(DE3)

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