› 7xov

Electron Microscopy
3Å resolution

Structural insights into human brain gut peptide cholecystokinin receptors

Released:
DOI: 10.2210/pdb7xov/pdb
PDB entry 7xov coloured by chain, front view.
PDB entry 7xov coloured by chain, side view.
PDB entry 7xov coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into human brain-gut peptide cholecystokinin receptors.
Ding Y, Zhang H, Liao YY, Chen LN, Ji SY, Qin J, Mao C, Shen DD, Lin L, Wang H, Zhang Y, Li XM
OpenAccess logo Cell Discov 8 55 (2022)
PMID: 35672283
Related structures: EMD-33360

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225594 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.28 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 96%)
  • Best match: P63092-2 (Residues: 1-380)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 338 amino acids
Theoretical weight: 37.2 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 3-340; Coverage: 99%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 126 amino acids
Theoretical weight: 13.71 KDa
Source organism: synthetic construct
Expression system: Not provided
Cholecystokinin receptor type A Chain: R
Molecule details ›
Chain: R
Length: 428 amino acids
Theoretical weight: 47.89 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P32238 (Residues: 1-428; Coverage: 100%)
Gene names: CCKAR, CCKRA
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand IA1 1 x IA1
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-33360
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

3 citation in other articles

A Novel CCK Receptor GPR173 Mediates Potentiation of GABAergic Inhibition.
He et al. (2023)
2 more