7xrp

Electron Microscopy
3.88Å resolution

Cryo-EM structure of SARS-CoV-2 spike protein in complex with nanobody C5G2 (localized refinement)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-534661 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Spike protein S1 Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 32.19 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 27-309; Coverage: 23%)
Gene names: 2, S
Sequence domains: Betacoronavirus-like spike glycoprotein S1, N-terminal
C5G2 nanobody Chain: B
Molecule details ›
Chain: B
Length: 125 amino acids
Theoretical weight: 13.38 KDa
Source organism: Camelus dromedarius
Expression system: Escherichia coli
Spike protein S1 Chain: F
Molecule details ›
Chain: F
Length: 192 amino acids
Theoretical weight: 21.67 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 330-521; Coverage: 15%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.88Å
Relevant EMDB volumes: EMD-33416
Expression systems:
  • Homo sapiens
  • Escherichia coli